Movie of Growing Etiolated Columbia-0 Hypocotyls

Seedlings were treated with air (white background) for 1 hour followed by 2 hours of 10 mL L-1 ethylene (black background) followed by air for another 5 hours (white background). We found that loss-of-function mutations in the ethylene receptors have no effect on the onset kinetics of growth inhibition indicating overlapping roles for the receptors in growth inhibition. In contrast, loss-of-function mutations in ETR1, ETR2 and EIN4 (but not ERS1 or ERS2) resulted in longer times for growth recovery after the removal of ethylene. This delayed growth recovery phenotype was rescued by a wild-type genomic ETR1 transgene but not a kinase-inactivated form suggesting a role for phosphotransfer in growth recovery after treatment with ethylene (Binder et al., 2004). This likely involves signaling to the cytokinin pathway (Binder et al., 2018).