Movie of Growing Etiolated Columbia-0 Hypocotyls
Seedlings were treated with air (white background) for 1
hour followed by 2 hours of 10 mL L-1
ethylene (black background) followed by air for another 5 hours (white
background). We found that loss-of-function mutations in the ethylene receptors
have no effect on the onset kinetics of growth inhibition indicating overlapping
roles for the receptors in growth inhibition. In contrast, loss-of-function
mutations in ETR1, ETR2 and EIN4 (but not ERS1 or ERS2)
resulted in longer times for growth recovery after the removal of ethylene.
This delayed growth recovery phenotype was rescued by a wild-type genomic
ETR1 transgene but not a kinase-inactivated form suggesting a role for
phosphotransfer in growth recovery after treatment with ethylene (Binder et
al., 2004).
